Mouse IL-17D ELISA Kit (ab213869)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-17D ELISA Kit
See all IL-17D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 715pg/ml 30.03 4.2% 2 16 1410pg/ml 64.86 4.6% 3 16 2703ng/ml 137.9 5.1% Inter-assay Sample n Mean SD CV% 1 24 694pg/ml 38.17 5.5% 2 24 1582pg/ml 99.67 6.3% 3 24 2691pg/ml 180.3 6.7% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse IL-17D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213869) is designed for the quantitative measurement of Mouse IL-17D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-17D amount of sample captured in plate.
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Notes
IL-17D is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines, and it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse IL-17D coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse IL-17D antibody 1 x 100µl Lyophilized recombinant Mouse IL-17D standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. -
Tissue specificity
Expressed preferentially in adipose, skeletal muscle and CNS. -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 17D
- IL 22
- IL 27
see all -
Database links
- Entrez Gene: 239114 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213869 has not yet been referenced specifically in any publications.