Mouse IL-17RC ELISA Kit (ab213870)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-17RC ELISA Kit
See all IL-17RC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 184pg/ml 11.77 = 6.4% 2 16 1503pg/ml 60.12 = 4% 3 16 5668pg/ml 345.74 = 6.1% Inter-assay Sample n Mean SD CV% 1 24 190pg/ml 13.68 = 7.2% 2 24 1560pg/ml 65.52 = 4.2% 3 24 5820pg/ml 413.22 = 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse IL-17RC Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213870) is designed for the quantitative measurement of Mouse IL-17RC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17RC has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E22-R440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-17RC amount of sample captured in plate.
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Notes
Interleukin-17 receptor C is a protein that in humans is encoded by the IL-17RC gene. By genomic sequence analysis, IL-17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse IL-17RC coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse IL-17RC antibody 1 x 100µl Lyophilized recombinant Mouse IL-17RC standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Relevance
IL-17RC is a single pass transmembrane protein that shares limited similarity with the interleukin 17 receptor. It is widely expressed in non leukocyte tissues and forms a heteromeric receptor for IL-17 with IL-17RA. At least four different isoforms are known to exist. -
Cellular localization
Cell membrane; Single pass type I membrane protein. Note: soluble isoforms may be produced. -
Alternative names
- IL 17 receptor C
- IL 17RC
- IL 17RL
see all -
Database links
- Entrez Gene: 171095 Mouse
- SwissProt: Q8K4C2 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213870 has not yet been referenced specifically in any publications.