Mouse IL-2 ELISA Set (without plates) (ab47591)
Overview
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Product name
Mouse IL-2 ELISA Set (without plates)
See all IL-2 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
15.6 pg/ml - 500 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-2 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure murine IL-2 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The murine IL-2 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant Murine IL-2
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-murine IL-2 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml Murine IL-2 Capture antibody 2 x 0.1ml 1 x 0.1ml 3 x 0.1ml Murine IL-2 standard (Lyophilized) Yellow 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab47591 has been referenced in 2 publications.
- Li S et al. CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer. J Gastroenterol Hepatol 37:154-163 (2022). PubMed: 34734434
- Zhang L et al. c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis. Clin Transl Med 11:e464 (2021). PubMed: 34586738