Mouse IgG + IgM + IgA secondary antibody (ab8516)

Product Name 

Mouse IgG + IgM + IgA secondary antibody

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Mouse IgG + IgM + IgA

Species Reactivity 

None with albumin fraction of mouse serum.
None with albumin fraction of other animal species
No background was seen on human tissue sections.

Specificity 

Antibodies were raised in rabbit to mouse IgG, IgM and IgA

Tested applications 
(see key)

Dot, ELISA

Application notes 
(see key)

Recommended dilutions
Recommended dilution as secondary antibody in ELISA is 1:1000.
Recommended dilution as secondary antibody in dot-blotting is 1:100.

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Mouse >> IgG + IgM + IgA >> Unconjugated
Immunology >> Immunoglobulins >> Heavy Chain >> IgM
Immunology >> Immunoglobulins >> Heavy Chain >> IgA
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

IgG is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Phosphate MSDS

Purification notes 

Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Mouse IgG + IgM + IgA secondary antibody (ab8516)

  This product has been used in:

Schmid M et al. ChIC and ChEC; genomic mapping of chromatin proteins. Mol Cell 16:147-57 (2004). PubMed: 15469830

If you publish research using ab8516 please let us know so that we can cite the reference on this datasheet.

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