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- Secondary antibodies
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Product name
Goat anti-Mouse IgG + IgM H&L (FITC) secondary antibody
See all IgG + IgM products (20) ...
Description
Goat polyclonal Secondary Antibody to Mouse IgG+IgM - H&L (FITC), pre-adsorbed
Target species
Mouse
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM.
Tested applications
Flow Cyt, ICC/IFmore details
Minimal
cross-reactivity
Cow, Horse, Humanmore details
Immunogen
Full length native protein (purified) (Mouse)
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
This antibody was labelled with fluorescein isothiocyanate (FITC, MW 390 da) Fluorochrome/Protein Ratio: 3.8 moles FITC per mole of Goat IgGAbsorption Wavelength: 495 nm Emission Wavelength: 528 nm
Clonality
Polyclonal
Isotype
IgG
Research areas
Secondary antibodies >> anti-Mouse >> IgG + IgM >> Fluorophore >> FITC
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
ICC/IF
Application notes
Flow Cyt: 1/2000 - 1/10000.
IF (microscopy): 1/500 - 1/2500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat anti-Mouse IgG + IgM H&L (FITC) secondary antibody (ab47830)
This product has been referenced in:
- Li CY et al. CFTR is essential for sperm fertilizing capacity and is correlated with sperm quality in humans. Hum Reprod 25:317-27 (2010). ICC/IF.Read more (PubMed: 19923167) »
- Murphy MJ et al. Essential role for Pbx1 in corneal morphogenesis. Invest Ophthalmol Vis Sci 51:795-803 (2010).Read more (PubMed: 19797217) »
See all 3 publications for this product
Publishing research using ab47830? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"