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Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002)

Submit an Abreview Q&A (8)Specific References (1)

Overview

  • Product nameGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesMouse
  • Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr more details
  • Minimal
    cross-reactivity
    Cow, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Mouse IgG (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide, 0.01% Thimerisol
    Constituents: 0.01% Soduim Phosphate, 15mg/ml BSA, 0.25M Sodium Chloride. pH 7.6
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality Polyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/100 - 1/250.
Immunomicroscopy IM: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: 1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.
IHC-P IHC-P: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.

References for Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002)

This product has been referenced in:
  • Schulz U  et al. Interleukin-10 Promotes NK Cell Killing of Autologous Macrophages by Stimulating Expression of NKG2D Ligands. Scand J Immunol 72:319-31 (2010). Flow Cyt . Read more (PubMed: 20883317) »

See 1 Publication for this product

Publishing research using ab7002 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab7002 Submit a Question for ab7002

Displaying 1 - 8 of 8 results for Abreviews and Q&A

10
Votes: Highest First

thank you very much for this kind offer. As we plan to use the TLR4 antibody with rat and human cells we would like to use the ab91532 product as isotype control.

I have issued a free of charge replacement for one vial of ab91532. To check the status of the order please contact our Customer Service team and reference this number. Please be aware this product is currently out of stock. Expect delivery around Decembe...

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we are using your product nr ab7002 as isotype control for your TLR-4-antibody (nr.ab45104). As we want to identify TLR4 on monocytes we use an ED9-antibody, too. We know have a problem with the isotype control, which seems to bind to the ED-9-antibody, a...

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Thank you for contacting us. The product ab7002 is not an isotype control, but a secondary antibody that binds to antibodies raised in mouse (such as ab45104). I am not certain which ED9-antibody (ab34086 Anti-SIRP alpha antibody [ED9] ??) you are r...

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  I would like to ask, in order to be absolutely certain, whether your secondary antibodies, mentioned below, contain Fc part: Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002) Donkey F(ab')2 polyclonal Secondary An...

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Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy to o...

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Thank you for the informations. Is it possible to know approximately when you will have a new batch of this monoclonal Ab in stock? Also, as I told you, the experiment worked well at the beginning (with the first lot# we bought) but not so well wit...

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Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit to t...

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Hi, Several weeks ago, I've bought one of your mouse monoclonal antibody (aE11 to C5b-9). As a negative control, I've also bought an isotype control (mouse IgG2a kappa monoclonal (MG2a-53)) and a Goat polyclonal antibody to Mouse IgG PE (ab7002) as a ...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of the...

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I would kindly ask if it is possible to get a free sample of secondary antibody Cat. No. ab7002. We would like to use this antibody to analyze CK 20 (primary mouse antibody, Dako) in urothelial cells by flow cytometry and immunofluorescence microscopy. Th...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you de...

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Hello. I am interested in your product ab7002-500, goat polyclonal to mouse IgG RPE conjugated, or ab7000-500 (the same but made in rabbit). However, I need the antibody to be adsorbed against rat, so that it doesn't cross-react with rat IgG. You say t...

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ab7002 has minimized cross-reactivity with Bovine, Horse, Human, Rabbit and Swine serum proteins. Ab7000 has minimized cross-reactivity with Human serum proteins. Since rat IgG's are not on the list of either product, the antibodies (anti-Mouse) may quit...

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How much ab should I use in order to stain 2 x 10 8 cells ? Could you please provide me with some information about molecular weight and concentration of your ab ? Many thanks v. Bernuth

This product is 500 kDa if only one antibody is attached and the recommended amount is 1 microgram per 1 X 10(6) cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"