Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002)
- Product nameGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesMouse
- Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr more details
Cow, Hamster, Horse, Human, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Mouse IgG (purified).
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide, 0.01% Thimerisol
Constituents: 0.01% Soduim Phosphate, 15mg/ml BSA, 0.25M Sodium Chloride. pH 7.6
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
- Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
- Clonality Polyclonal
- General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
- Research Areas
Our Abpromise guarantee covers the use of ab7002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: 1/100 - 1/250.|
|Immunomicroscopy||IM: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: 1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
References for Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002)
This product has been referenced in:
- Schulz U et al. Interleukin-10 Promotes NK Cell Killing of Autologous Macrophages by Stimulating Expression of NKG2D Ligands. Scand J Immunol 72:319-31 (2010). Flow Cyt . Read more (PubMed: 20883317) »