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Donkey anti-Mouse IgG H&L (FITC) secondary antibody (ab7057)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product nameDonkey anti-Mouse IgG H&L (FITC) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (FITC), pre-adsorbed
  • Target speciesMouse
  • Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse whole (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/500 - 1/2500.
Immunomicroscopy IM: Use at an assay dependent concentration.
Flow Cyt Flow Cyt: 1/2000 - 1/10000.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.

References for Donkey anti-Mouse IgG H&L (FITC) secondary antibody (ab7057)

This product has been referenced in:
  • Miranda E  et al. The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Hum Mol Genet 17:1527-39 (2008). Read more (PubMed: 18267959) »
  • Griffin S  et al. Astrocyte-neurone communication following oxygen-glucose deprivation. J Neurochem 95:1015-22 (2005). Read more (PubMed: 16190880) »

See all 2 Publications for this product

Publishing research using ab7057 ? Please let us know so that we can cite the reference in this datasheet

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Dear Abcam

Does your antibody Donkey-anti-mouse IgG FITC (ab7057) come from the same donkey as either of the following antibodies:



Donkey-anti-mouse IgG FITC (Jackson 715-095-151)

Donkey anti-mouse IgG FITC (Mi...

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Thank you for your enquiry.

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product (ab7057) falls into the latter category. The identity of ou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"