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Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642)See all IgG secondary antibodies ...
  • Target speciesMouse
  • Specificityab60318 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IF more details
  • Immunogen

    Mouse IgG.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesChromeo™ 642 is spectrally similar to Cy5®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 642 and 666 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 180,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 7 % Excitation Wavelength Range: 630 to 655 nm Emission Wavelength Range: 650 to 670 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
  • Application notesICC/IF: 1/1000 - 1/2000.
    Plate-based assays: 1/800 to 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) images

    • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
      Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
      ab60318 staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense with a DAPI nuclear counterstain.

    References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)

    ab60318 has not yet been referenced specifically in any publications.

    Publishing research using ab60318 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab60318 Submit a Question for ab60318

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Thank you for the attention,
    we have tried to use others primary antibodies and the results are always the same, so I accept your proposal to replace the antibodies,
    could I know approximate consignment date of new antibodies?

    thank y...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry these secondary antibodies did not perform as stated on the datasheet and for the inconve...

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    i have attached the completed questionaire

    Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

    I appreciate the time you have spent in the laboratory and understand...

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    Hello,
    I'm Annunziata Crupi, PhD Student in DAHFMO, histology and medical embriology unit, La Sapienza, Rome, laboratory of M. Bouchè,
    I have some problems with two abcam conjugated antibodies and I have attached a file that explain every...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondary antibodies.

    I appreciate the time you have spent in the laboratory and understand your concerns. It ...

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    compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

    When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"