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Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6727)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin)See all IgG secondary antibodies ...
  • Target speciesMouse
  • Tested applicationsDot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB more details
  • Immunogen

    Mouse IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
ICC/IF
WB
  • Application notesimmunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency

    • References for Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6727)

    ab6727 has not yet been referenced specifically in any publications.

    Publishing research using ab6727 ? Please let us know so that we can cite the reference in this datasheet

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    Is it azide-free ?

    This antibody preparation contains 0.01% (w/v) Thimerisol as preservative; it does not contain azide.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"