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Immunocytochemistry/ Immunofluorescence - Mouse IgG antibody (ab60316)
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Product Name
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Mouse IgG secondary antibody
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Product type
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Secondary antibodies
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Description
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Goat polyclonal to Mouse IgG (Chromeo™ 546)
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Reacts with
(species key)
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Ms
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Specificity
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ab60316 reacts with whole molecular mouse IgG and the light
chains of other mouse immunoglobulins. No cross-reactivity with
non-immunoglobulin serum proteins observed.
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Tested applications
(see key)
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ICC/IF
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1000 - 1/2000.
Plate-based assays: 1/800 to 1/1000
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> Chromeo™ 546
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 16017 (Mouse)
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Conjugation notes
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Chromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets.
Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax)
Quantum Yield: ~ 11 %
Excitation Wavelength Range: 530 to 550 nm
Emission Wavelength Range: 560 to 570 nm
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Storage buffer
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Preservative: 2mM Sodium Azide
Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Chromeo™ 546
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mouse IgG secondary antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Mouse IgG secondary antibody images:
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 Immunocytochemistry/ Immunofluorescence - Mouse IgG antibody (ab60316)
EGF receptor detected in an urothel cell line by ICC/IF using ab60316 at 1/1000. Paraffin-treated tissue.
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Search PubMed (MEDLINE) for references to IgG
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Mouse IgG secondary antibody - more information
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Customer reviews (feedback) regarding Mouse IgG secondary antibody |
Customer FAQs regarding Mouse IgG secondary antibody |
Protocols for Mouse IgG secondary antibody |
Price and availability of products related to Mouse IgG secondary antibody |
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Mouse IgG secondary antibody - other tools:
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Contact Abcam with a Technical Enquiry about Mouse IgG secondary antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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