Mouse IgG1 [B11/6] - Isotype Control (ab91353)

Overview

  • Product name
    Mouse IgG1 [B11/6] - Isotype Control
    See all Mouse isotype controls
  • Specificity
    This mouse monoclonal IgG1 was produced against a synthetic hapten, which is normally not present in humans or animals. Therefore, this mouse IgG1 does not react with any antigen of human or animal cells.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse. Synthetic hapten

  • General notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab91353 is purified and passed through a 0.22µm filter
  • Isotype control notes
    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
  • Clonality
    Monoclonal
  • Clone number
    B11/6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91353 is a negative isotype control
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Mouse IgG1 [B11/6] - Isotype Control images

  • Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Mouse IgG1 [B11/6] - Isotype Control (ab91353)

This product has been referenced in:
  • Ring KL  et al. Potential immunotherapy targets in recurrent cervical cancer. Gynecol Oncol N/A:N/A (2017). IHC . Read more (PubMed: 28233576) »
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). Read more (PubMed: 27924000) »

See all 12 Publications for this product

Product Wall

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please ...

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Thank you for contacting us.
I can confirm the isotype controls suggested are suitable for the antibodies requested.
The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s vo...

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Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab91353 (Abcam does not issue Certificate of Analysis).

If there is anything else I can help you with, please let me know.

I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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Thank you for contacting Abcam.

I am sorry that you are having some issues using ab668 in flow cytometry. The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on host of samples. If we cannot ...

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Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...

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I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-7...

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Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just ...

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Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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