Mouse IgG1 [ICIGG1] - Isotype Control (ab91353)

Overview

  • Product nameMouse IgG1 [ICIGG1] - Isotype ControlSee all Mouse isotype controls ...
  • Specificityab91353 reacts with undefined epitope on a plant pathogen. No reactivity with cell surface antigens on Human.
  • Tested applicationsFlow Cyt, IHC-P, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse. vegetal protein

  • General notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab91353 is purified and passed through a 0.22µm filter
  • Isotype control notes ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
  • ClonalityMonoclonal
  • Clone numberICIGG1
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91353 is a negative isotype control
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References for Mouse IgG1 [ICIGG1] - Isotype Control (ab91353)

This product has been referenced in:
  • Marion-Poll L  et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt . Read more (PubMed: 24713002) »
  • Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . Read more (PubMed: 24573683) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.
I can confirm the isotype controls suggested are suitable for the antibodies requested.
The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s vo...

Read More

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

Read More

Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab91353 (Abcam does not issue Certificate of Analysis).

If there is anything else I can help you with, please let me know.

I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

Read More

Thank you for contacting Abcam.

I am sorry that you are having some issues using ab668 in flow cytometry. The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on host of samples. If we cannot ...

Read More

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...

Read More

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-7...

Read More

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just ...

Read More

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please ...

Read More

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"