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Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)

Q&A (6)Specific References (1)

Overview

  • Product nameMouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide FreeSee all Mouse isotype controls ...
  • SpecificityThis mouse monoclonal IgG1 was produced against a synthetic hapten, which is normally not present in humans or animals. Therefore, this mouse IgG1 does not react with any antigen of human or animal cells.
  • Tested applicationsWB, IP, ELISA, IHC-P, IHC-Fr, Gel supershift assays, Flow Cyt, ICC/IF more details
  • Species reactivity
    Does not react with any antigen of human or animal cells.
  • Immunogen

    Synthetic hapten.

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis monoclonal IgG1 was purified from mouse ascites.
  • Clonality Monoclonal
  • Clone numberNCG01
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab81032 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    IP
    ELISA
    IHC-P
    IHC-Fr
    Gel supershift assays
    Flow Cyt
    ICC/IF
  • Application notesNegative Control for:

    ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    GSA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)

    This product has been referenced in:
    • Ma J  et al. The M1 form of tumor-associated macrophages in non-small cell lung cancer is positively associated with survival time. BMC Cancer 10:112 (2010). IHC-P . Read more (PubMed: 20338029) »

    See 1 Publication for this product

    Publishing research using ab81032 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab81032

    Displaying 1 - 6 of 6 results for Q&A

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    I recently conducted an experiment using a range of abcam monoclonal antibodies using the following as an isotype control to check I do not have non specific binding.

    Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)<...

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    Thank you for contacting us.

    We have sourced this antibody from external collaborator laboratory. Unfortunately they have not released this information to us so I am sorry we are unable to help in this case.

    I hope this information is ne...

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    For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
    ab36204 --> Rabbit IgG
    ab48614 --> Rabbit IgG
    ab66219 --> Rab...

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    Thank you for your clarification.

    I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I c...

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    Good day
    I have a question about ab81032 product - Negative control IgG1.We had bought this Negative control IgG1 post-sale.
    This IgG1 can’t attache to anywhere and dosen’t give any signals.
    We had used MABA4 in normal reaction - this antib...

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    Thank you for contacting us.

    I am sorry to hear you are having unsatisfactory results with one of our products.

    Ab81032 is an isotype control, therefore it doesn’t recognise any target of Human or animal cells. In your email you ...

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    Greetings -
    I wouldkindly ask you tosee the attached files (customer complaint).
    Thank you in advance for help in this issue.
    Best regards,

    Thank you for contacting us and sending us the protocol and the images. I am sorry to hear your customer is having problems with one of our products.

    Our policy is that we will offer pre- and post-sale technical support for products sold via una...

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    Do you know the purity of this antibody and how do you quantify the concentration?

    Thank you for contacting us. My colleague is out today but we did recieve information regarding your enquiry so I thought I would pass than on to you. According to the lab, we do not quantify the purity of this product. The product is purified from ascite...

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    Do you know the purity of this antibody and how do you quantify the concentration?

    Thank you for contacting Abcam. The only information that I can find out about the purity of ab81032 is that it was purified from ascites fluid by Protein G chromatogrphy, I cannot find out any information about how pure the final product is. I am sorry ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"