Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)

Overview

  • Product nameMouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free
    See all Mouse isotype controls
  • SpecificityThis mouse monoclonal IgG1 was produced against a synthetic hapten, which is normally not present in humans or animals. Therefore, this mouse IgG1 does not react with any antigen of human or animal cells.
  • Tested applicationsWB, IP, ELISA, IHC-P, IHC-Fr, Gel supershift assays, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Does not react with any antigen of human or animal cells.
  • Immunogen

    Synthetic hapten.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis monoclonal IgG1 was purified from mouse ascites.
  • Clonality Monoclonal
  • Clone numberNCG01
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab81032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
IHC-P
IHC-Fr
Gel supershift assays
Flow Cyt
ICC/IF
  • Application notesNegative Control for:

    ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    GSA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Mouse IgG1 Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)

    This product has been referenced in:
    • Takano K  et al. Characteristic expressions of GABA receptors and GABA producing/transporting molecules in rat kidney. PLoS One 9:e105835 (2014). Read more (PubMed: 25188493) »
    • Meredith JE  et al. Characterization of Novel CSF Tau and ptau Biomarkers for Alzheimer's Disease. PLoS One 8:e76523 (2013). WB . Read more (PubMed: 24116116) »

    See all 3 Publications for this product

    Product Wall

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    We have sourced this antibody from external collaborator laboratory. Unfortunately they have not released this information to us so I am sorry we are unable to help in this case.

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    Thank you for your clarification.

    I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...

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    I am sorry to hear you are having unsatisfactory results with one of our products.

    Ab81032 is an isotype control, therefore it doesn’t recognise any target of Human or animal cells. In your email y...

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    Thank you for contacting us and sending us the protocol and the images. I am sorry to hear your customer is having problems with one of our products.

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    Thank you for contacting us. My colleague is out today but we did recieve information regarding your enquiry so I thought I would pass than on to you. According to the lab, we do not quantify the purity of this product. The product is purified from asc...

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    Thank you for contacting Abcam. The only information that I can find out about the purity of ab81032 is that it was purified from ascites fluid by Protein G chromatogrphy, I cannot find out any information about how pure the final product is. I am sorr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"