FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control (ab106163)
Related conjugates and formulations
Overview
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Product name
FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control
See all Mouse isotype controls -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Conjugation notes
Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. -
Specificity
ab106163 (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues. -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details -
General notes
ab106163 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
> 95% (by SDS-PAGE) -
Clonality
Monoclonal -
Clone number
MOPC-21 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Alternative Versions
- PerCP/Cy5.5® Mouse IgG1, Kappa Monoclonal [MOPC-21] - Isotype Control (ab157226)
- Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
- FITC Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype control (ab18435)
- Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)
- AlexaFluor ® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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IP |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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Flow Cyt |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
IP
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Flow Cyt
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab106163 has been referenced in 8 publications.
- Gałdyszyńska M et al. The stiffness-controlled release of interleukin-6 by cardiac fibroblasts is dependent on integrin α2β1. J Cell Mol Med 24:13853-13862 (2020). PubMed: 33124775
- Guo X et al. Hepatitis C virus infection induces endoplasmic reticulum stress and apoptosis in human fetal liver stem cells. J Pathol N/A:N/A (2019). PubMed: 30680725
- Avril M et al. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption. Sci Rep 9:13142 (2019). PubMed: 31511575
- Mao X et al. The rescue effect of mesenchymal stem cell on sodium iodate-induced retinal pigment epithelial cell death through deactivation of NF-?B-mediated NLRP3 inflammasome. Biomed Pharmacother 103:517-523 (2018). PubMed: 29677537
- Smed-Sörensen A et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood 111:5037-46 (2008). PubMed: 18337560
- Yates J et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol 19:785-99 (2007). PubMed: 17545278
- Carlsten M et al. DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res 67:1317-25 (2007). PubMed: 17283169
- Bryceson YT et al. Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med 202:1001-12 (2005). PubMed: 16203869