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Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control (ab18435)

Q&A (5)

Overview

  • Product nameMouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype controlSee all Mouse isotype controls ...
  • ConjugationFITC
  • Conjugation notesThe solution is free of unconjugated FITC.
  • Tested applicationsFlow Cyt more details
  • Immunogen

    KLH

  • General notesIsotype control has been screened on a variety of resting, activated, live, and fixed rat and human tissues.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Monoclonal
  • Clone numberMOPC-21
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18435 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt Flow Cyt: Use at an assay dependent dilution. (surface or intracellular staining)

    References for Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control (ab18435)

    ab18435 has not yet been referenced specifically in any publications.

    Publishing research using ab18435 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab18435

    Displaying 1 - 5 of 5 results for Q&A

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    Votes: Highest First

    Customer kindly contacted us to determine the concetration of Anti-CD34 antibody [4H11[APG]] (FITC) so they may use the appropriate amount of isotype control in Flow Cytometry.

    Thank you for contacting us.

    Unfortunately we have not determined the exact concentration for this product. Before conjugation with FITC this product has a 1mg/ml concentration. When using isotype controls in Flow Cytometry keep in mind that ...

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    Dear Sir / Madam,
    our customer was previously using these 2 items for his experiments:
    AB86886 Abcam Anti-CD4 antibody [RPA-T4] (FITC) (100 ug)
    AB18435 Abcam Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype
    control, 200 µg...

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    Thank you very much for your inquiry.

    Yes, you are absolutely correct. I can confirm, that the non- conjugated equivalent of the ab86886 is the ab86885.

    When using an unconjugated antibody I can however also recommend to use an unconju...

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    Dear Sir / Madam,
    thank you for your reply,
    my customer would like to order:
    ab51387 Product nameAnti-CD4 antibody [RFT-4g] (Phycoerythrin -Texas Red®).
    May I know if the recommended isotype control would be Mouse IgG1 FITC
    co...

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    Thank you very much for your inquiry.

    For the ab51387 I recommend to use an isotype control which is of the same isotype and conjugation. I can therefore not recommend to use the ab18435 for this antibody as a control.

    We do currently ...

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    A customer wishes to get an isotype control for the above antibody and I suggested:
    AB37356 Abcam Mouse IgG (FITC) - Isotype Control (100 ug)
    However, the customer thinks it might not be suitable and ab18435 Mouse IgG1, kappa monoclonal [MOPC-21...

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    The anti-CD4 antibody [RPA-T4] (FITC), ab86886, is a Mouse IgG1. The best isotype control for ab86886 would be a Mouse IgG1 FITC conjugated such as ab18435.

    ab37356 would be a good isotype control for a Mouse polyclonal IgG FITC conjug...

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    Regarding the following product: http://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MOPC-21-FITC-Isotype-control-ab18435.html Please could you tell me the price and if the product was amplified in tissue culture supernatant or in animal ascites. Many than...

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    Thank you for contacting us. Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control, ab18435, is produced in ascites. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"