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Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

Q&A (2)Specific References (5)

Overview

  • Product nameMouse IgG1, kappa monoclonal [MOPC-21] - isotype controlSee all Mouse isotype controls ...
  • Specificityab18443 MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.
  • Tested applicationsIHC-P, IHC-Fr, Flow Cyt, IP, WB more details
  • Immunogen

    N/A

  • General notes


    ab18433 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Isotype control notes ab18433 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality Monoclonal
  • Clone numberMOPC-21
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18443 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P
    IHC-Fr
    Flow Cyt
    IP
    WB
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

    This product has been referenced in:
    • Pohar J  et al. The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists. J Biol Chem 288:442-54 (2013). Flow Cyt ; Human . Read more (PubMed: 23166319) »
    • Schmidt H  et al. Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells. Cell Commun Signal 9:4 (2011). Read more (PubMed: 21255389) »

    See all 5 Publications for this product

    Publishing research using ab18443 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab18443

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    We purchased your IgG 1 Kappa control antibody (ab18443) about 4 months ago. I was wondering if you could tell me what the lot number would be for that timeframe.

    We accidentally tossed the vial and I need it for a report I'm filling out. Thank...

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    Thank you for contacting us.

    If you provide me with the institution that placed the order, I will be able to tell you the exact lot number you purchased. However, roughly 4 months ago we were selling lot GR54839-4.

    I hope this inform...

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    Need IgG isotype control for IHC on paraffin sections. I have 3 mouse monoclonal antibodies which I require isotype controls for - 2 mouse are type IgG1, one is IgG1,kappa. Do I need to match for the kappa as well or can I just use a IgG1? Please ca...

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    Thank you for your enquiry. According to my literature 99% of mouse IgG immunoglobulin molecules have a kappa light chain. This is reflected in the number of IgG, kappa isotype controls in our catalogue. Therefore given that your three mouse antibodie...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"