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Mouse IgG2a (FITC) - Isotype control (ab81197)

Q&A (5)Specific References (1)

Overview

  • Product nameMouse IgG2a (FITC) - Isotype controlSee all Mouse isotype controls ...
  • ConjugationFITC
  • Conjugation notesExcitation: 488 nm. Peak emission: 525 nm.
  • SpecificityThis mouse isotype control is intended for use to identify the amount of nonspecific binding of mouse monoclonal antibodies on human cells by flow cytometric methods.
  • Tested applicationsFlow Cyt more details
  • General notesReagents should be brought to room temperature (22 ± 3°C) before use. Protect cells from light sources during incubation with antibodies and prior to analysis.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 4mg/ml BSA, 0.3M Sodium chloride, 0.02M Sodium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab81197 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt Flow Cyt: Use at an assay dependent dilution.

    References for Mouse IgG2a (FITC) - Isotype control (ab81197)

    This product has been referenced in:
    • Morrison VL  et al. TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection. J Immunol 185:2737-46 (2010). Flow Cyt ; Mouse . Read more (PubMed: 20660707) »

    See 1 Publication for this product

    Publishing research using ab81197 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab81197

    Displaying 1 - 5 of 5 results for Q&A

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    Votes: Highest First

    Dear Tech Support Team,
    Kindly advise if the following isotypes controls are the compatible options for the mentioned antibodies:
    Isotype control for ab6142 anti-Nestin unconjugated
    http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://w...

    Read More

    Thank you for contacting us.
    I can confirm the isotype controls suggested are suitable for the antibodies requested.
    The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s volum...

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    Is the concentration of FITC in ab58864 40x as in Ab81197 ( IgG2a Isotype)?

    I checked the TLR9 antibody list. Is the Anti-TLR9 antibody [5G5] (ab16892) exactly same as ab58864 except FITC labeling? However, it does not react with human cells. D...

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    Thank you for your reply.

    Although the exact amount of FITC in ab58864 and ab81197 has not been determined, for the purposes of your calculations, you can assume that it is 40x greater.

    Both ab16892 and ab58864, are the same clone of a...

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    Hi, Anti-TLR9 antibody [5G5] (FITC) (ab58864) worked very good in our expt. Now question is about FITCIgG2a isotype control (ab81197). What is the concentration? What is the dilution if we use it as control for ab58864. Besides, can we purchase the...

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    Thank you for contacting us.

    Ab81197 Mouse IgG2a (FITC) - Isotype control is supplied in a500 µl size at 4mg/ml. To correctly use this control you will need to use this at the same concentration as yourab58864 Anti-TLR9 antibody [5G5] (...

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    Hi,
    do you have this product (ab106166) in stock?
    Thank you

    Hello! Yes, this product is in stock. You can also search our website for the catalog number and the top of the datasheet will tell you whether it is in stock. Please let me know if you have any further questions!

                        Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format          ...

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    Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"