Overview
Vegetal protein
Properties
Constituents: 1% BSA, PBS, pH 7.2
Concentration information loading...-
Alternative names
- Mouse Isotype Control
Applications
Our Abpromise guarantee covers the use of ab91361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt: Use 1µg for 106 cells. ab91361 is a negative isotype control |
Mouse IgG2a [ICIGG2A] - Isotype Control images
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Flow Cytometry - Anti-MUC4 antibody (ab60720)Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Mouse IgG2a [ICIGG2A] - Isotype Control (ab91361)
ab91361 has not yet been referenced specifically in any publications.
