Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414)

Overview

  • Product nameMouse IgG2a, kappa monoclonal [MG2a-53] - Isotype controlSee all Mouse isotype controls ...
  • Tested applicationsFunctional Studies, Flow Cyt, WB, IP, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    ab18414, MG2a-53 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesAb18414 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.
  • Isotype control notes ab18414, MG2a-53 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality Monoclonal
  • Clone numberMG2a-53
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Flow Cyt
WB
IP
IHC-Fr
IHC-P
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    FuncS: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414)

    This product has been referenced in:
    • Su SC  et al. Annexin 2 Regulates Endothelial Morphogenesis by Controlling AKT Activation and Junctional Integrity. J Biol Chem 285:40624-34 (2010). Read more (PubMed: 20947498) »

    See 1 Publication for this product

    Product Wall

    I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

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    Thank you for your enquiry.

    I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

    The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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    I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

    DISCOUNT CODE: ######

    Expiration date: ######

    This code will give you: 1 free primary antibody before the expiration date. To redee...

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    Thank you for contacting us.

    We do not currently have a pan- mouse IgG isotype control suitable for in vivo use on mouse. We do have isotype controls for each IgG subtype which have beenLEAF Purified. This is a low endotoxin azide free for...

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    Thank you for contacting us.


    Please check the following link

    http://www.abcam.com/Mouse-IgG2a-kappa-MOPC-173-low-endotoxin-isotype-control-ab18449.html

    http://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-co...

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    Clone MG2a-53 (mouse IgG2a isotype control) is originally derived from BALB/c.

    Thank you for your enquiry. We do not have specific data on the half-life of this antibody, but I can give you some numbers of rat IgG2b half-life based on literature. These references refer to natural IgG2 with a half-life of 20-30 days in bl...

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    Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit t...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"