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Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414)

Q&A (9)Specific References (1)

Overview

  • Product nameMouse IgG2a, kappa monoclonal [MG2a-53] - Isotype controlSee all Mouse isotype controls ...
  • Tested applicationsFunctional Studies, Flow Cyt, WB, IP, IHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Chemical / Small Molecule (Trinitrophenol) conjugated to KLH.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesAb18414 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.
  • Isotype control notes ab18414, MG2a-53 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality Monoclonal
  • Clone numberMG2a-53
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18414 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    Flow Cyt
    WB
    IP
    IHC-Fr
    IHC-P
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    FuncS: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414)

    This product has been referenced in:
    • Su SC  et al. Annexin 2 Regulates Endothelial Morphogenesis by Controlling AKT Activation and Junctional Integrity. J Biol Chem 285:40624-34 (2010). Read more (PubMed: 20947498) »

    See 1 Publication for this product

    Publishing research using ab18414 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab18414

    Displaying 1 - 9 of 9 results for Q&A

    10
    Votes: Highest First

    Hello  we are interested in participating the AbTrial program We will purchase two antibodies this time:  1. Sprouty-1: Ab56670 2. Sprouty-2: Ab60719  

    I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cytom...

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    Dear,
    I ordered the following antibodies and received them at the end of September:
    - Ab54426 (anti-CD62P)
    - Ab91353 (Mouse IgG1 – isotype control)
    - Ab 18981 (anti-CD62E)
    - Ab27478 (rabbit polyclonal IgG)
    - Ab2213 (ant...

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    Thank you for your enquiry.

    I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

    The others I would recommend to aliquot and freeze immediately. Keeping them at 4oC ...

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    Dear,
    We want to proceed and test ab54426 in IHC-P. You can send me the discount code.
    Are these the correct isotype antibodies for the primary antibodies I have chosen:
    - Mouse IgG2a, kappa monoclonal [MG2a-53] - isotype control (Ab18414) ...

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    I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

    DISCOUNT CODE: ######

    Expiration date: ######

    This code will give you: 1 free primary antibody before the expiration date. To redeem t...

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    Inquiry: I am looking for a mouse IgG antibody to use as an isotype control for in vivo mouse experiments. We would need 10mg total. Do you have anything suitable for in vivo experiments. If so, could I get a quote? Thank you very much,

    Thank you for contacting us.

    We do not currently have a pan- mouse IgG isotype control suitable for in vivo use on mouse. We do have isotype controls for each IgG subtype which have beenLEAF Purified. This is a low endotoxin azide free formul...

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    Could you please let me know if you have mouse IgG2a isotype control sodium azide free available?

    Thank you heaps J

    Kind regards,

    Thank you for contacting us.


    Please check the following link

    http://www.abcam.com/Mouse-IgG2a-kappa-MOPC-173-low-endotoxin-isotype-control-ab18449.html

    http://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-contr...

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    I have an inquiry regarding the Mouse IgG2a, kappa monoclonal [MG2a-53] Isotype control. Which mouse strain is this Ab raised in?

    Clone MG2a-53 (mouse IgG2a isotype control) is originally derived from BALB/c.

    Can you tell me how long it will take for the amount of mouse IgG to decrease till 50%, when injected into the blood stream of rats?

    Thank you for your enquiry. We do not have specific data on the half-life of this antibody, but I can give you some numbers of rat IgG2b half-life based on literature. These references refer to natural IgG2 with a half-life of 20-30 days in blood...

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    Thank you for the informations. Is it possible to know approximately when you will have a new batch of this monoclonal Ab in stock? Also, as I told you, the experiment worked well at the beginning (with the first lot# we bought) but not so well wit...

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    Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit to t...

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    Hi, Several weeks ago, I've bought one of your mouse monoclonal antibody (aE11 to C5b-9). As a negative control, I've also bought an isotype control (mouse IgG2a kappa monoclonal (MG2a-53)) and a Goat polyclonal antibody to Mouse IgG PE (ab7002) as a ...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"