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Mouse IgG2a, kappa [MOPC-173] (biotin) - Isotype control (ab18454)

Q&A (1)

Overview

  • Product nameMouse IgG2a, kappa [MOPC-173] (biotin) - Isotype controlSee all Mouse isotype controls ...
  • ConjugationBiotin
  • Specificityab18454 MOPC-173 immunoglobulin has unknown specificity. ab18454 MOPC-173 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • Tested applicationsFlow Cyt more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab18454 antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
  • Isotype control notes ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality Monoclonal
  • Clone numberMOPC-173
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18454 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface or intracellular staining.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG2a, kappa [MOPC-173] (biotin) - Isotype control (ab18454)

    ab18454 has not yet been referenced specifically in any publications.

    Publishing research using ab18454 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab18454

    Displaying 1 - 1 of 1 results for Q&A

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    Dear Abcam,
    I would like to ask a question about which control IgG I need to use for
    28-14-8 (biotin) (#ab25237)
    I want to use same isotype with biotin conjugated antibody and I found that ab 128976, which is biotinylated
    Goat anti-Mou...

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    Thank you for contacting us.

    I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

    I would recommend ab18454 as an isotype co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"