Mouse IgG2b [PLPV219] - Isotype Control (ab91366)

Overview

  • Product nameMouse IgG2b [PLPV219] - Isotype ControlSee all Mouse isotype controls ...
  • Specificityab91366 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91366 does not react with any antigen of Human or animal cells.
  • Tested applicationsFlow Cytmore details
  • Immunogen

    Vegetal protein

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberPLPV219
  • IsotypeIgG2b
  • Research Areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91366 is a negative isotype control

Mouse IgG2b [PLPV219] - Isotype Control images

  • Overlay histogram showing HeLa cells stained with ab54944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54944, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Mouse IgG2b [PLPV219] - Isotype Control (ab91366)

This product has been referenced in:
  • Carilho Torrao RB  et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Flow Cyt . Read more (PubMed: 24007191) »

See 1 Publication for this product

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Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. Howeve...

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