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Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control (ab18469)

Q&A (3)

Overview

  • Product nameMouse IgG2b, kappa monoclonal [MPC-11] - Isotype controlSee all Mouse isotype controls ...
  • Tested applicationsIHC-P, IHC-Fr, WB, Flow Cyt, IP more details
  • Immunogen

    Trinitrophenol and KLH.

  • General notesIsotype control has been screened on a variety of resting, activated, live, and fixed rat and human tissues.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Monoclonal
  • Clone numberMPC-11
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P
    IHC-Fr
    WB
    Flow Cyt
    IP
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface and intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control (ab18469)

    ab18469 has not yet been referenced specifically in any publications.

    Publishing research using ab18469 ? Please let us know so that we can cite the reference in this datasheet

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    I have found two mouse IgG2b isotype controls which I think are suitable for my IHC-P experiments on human endobronchial biopsies, but I have a question about them:
    -ab18421 clone MG2b-57 - immunogen used is Trinitrophenol + KLH, and the AB is said to...

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    Thank you for contacting Abcam.

    We have not tested in-house reactivity of ab18469 against KLH, so I cannot say for certain whether the antibody will or will not bind to the KLH tag.

    We have also not epitope mapped these antibodies, so we...

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    Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. A...

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    Thank you for your enquiry.


    Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize...

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    Dear Sir or Madam:

    I would like to have the original reference of the isolation of hybridoma clone IgG2b MPC-11 (cat#ab18469).

    Thank you for contacting us.

    The MPC-11 myeloma is desribed in the following paper:

    J Exp Med. 1970 Mar 1;131(3):515-41.
    Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin pl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"