You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Mouse IgG3, kappa monoclonal [MG3-35] - Isotype control (ab18394)

Q&A (5)Specific References (1)

Overview

  • Product nameMouse IgG3, kappa monoclonal [MG3-35] - Isotype controlSee all Mouse isotype controls ...
  • Tested applicationsFlow Cyt, WB, IP, IHC-P, IHC-Fr more details
  • Immunogen

    Full length protein (Trinitrophenol + KLH).

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Isotype control notes Ab18394 reacts with KLH. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality Monoclonal
  • Clone numberMG3-35
  • IsotypeIgG3
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Mouse Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18394 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt
    WB
    IP
    IHC-P
    IHC-Fr
  • Application notesFlow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Mouse IgG3, kappa monoclonal [MG3-35] - Isotype control (ab18394)

    This product has been referenced in:
    • Mitchell J & Sullam PM Streptococcus mitis phage-encoded adhesins mediate attachment to {alpha}2-8-linked sialic acid residues on platelet membrane gangliosides. Infect Immun 77:3485-90 (2009). Read more (PubMed: 19506011) »

    See 1 Publication for this product

    Publishing research using ab18394 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab18394

    Displaying 1 - 5 of 5 results for Q&A

    10
    Votes: Highest First

    We recently received a shipment of ab18394 and need to assign an expiration date to the raw material.

    Thank you for contacting Abcam.

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage...

    Read More

    I need a Certificate of Analysis for both lots of material shipped to me today.

    Please e-mail them as soon as possible so we can process the shipment.

    Thank you for contacting Abcam regarding ab18394.


    Attached please find the document you requested.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Thank you for your reply.  First of all, kindly advise the pattern of the run that the customer should observe with the isotype control.   Secondly, please read the attached correspondence regarding the compatibility of ab18394 with IP and Invitrogen's...

    Read More

    Thank you for contacting us.

    I did read over the specifications for dynabeads and I did not realize that they claim to have strong binding with IgG3 as most protein A beads have a weak binding with that particular isotype. However, because the pr...

    Read More

    Description of the problem: in isotype control lane of the IP performed there is no typical strong IgG heavy chain band observed in the experiment lane   Sample Nuclear extract digested with MNase Sample preparation incubation of sample with IP Ab’ ON ...

    Read More

    Thank you for contacting us and thank you for providing the protocol and image.
    After reviewing the data presented I would like to suggest a few changes that may help improve your results.

    Mouse IgG3 does not bind well to beads for IP. To ov...

    Read More

    Hi, Do you know the IgG concentration of isotypektr. Mouse IgG3,kappa, ab 18394? Best regards  

    Thank you for contacting us. The concentration of this product is 0.500 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"