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Product Name
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Mouse IgM secondary antibody
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Mouse IgM
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Immunogen
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Rabbits were immunized with highly purified normal mouse IgM in Freund’s adjuvant.
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Specificity
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The affinity purified anti IgM mu antibodies only react with IgM by immunodiffusion and Immunoelectrophoretic techniques.
A 1 mg/ml solution of the antibody is reacted against normal mouse serum and IgG1, IgG2a, IgG2b, IgG3, IgM and IgA.
By ELISA, there is minimal reactivity to IgG.
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Tested applications
(see key)
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ELISA, Ie, WB
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent concentration.
Ie: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Isoform 1: Secreted. During differentiation, B-lymphocytes switch from expression of membrane-bound IgM to secretion of IgM.
Isoform 2: Cell membrane; Single-pass type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Secondary antibodies >> anti-Mouse >> IgM >> Unconjugated
Immunology >> Immunoglobulins >> Heavy Chain >> IgM
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Relevance
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IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.
Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type.
Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, pH7.2
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Purification notes
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The antiserum was solid phase absorbed to make it IgM mu chain specific and the anti mu chain antibodies were isolated by solid phase immunoaffinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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