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Goat anti-Mouse IgM mu chain (HRP) secondary antibody (ab5930)

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Overview

Product name

Goat anti-Mouse IgM mu chain (HRP) secondary antibody
See all IgM products (217) ...

Description

Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP)

Target species

Mouse

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Tested applications

ICC/IF, IHC-Fr, Dot Blot, WB, ELISA, Electron Microscopy, IHCmore details

Immunogen

Mouse IgM mu chain

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Thimerosal
Constituents: 15mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.01M Sodium Phosphate. pH 7.6

Concentration

Concentration information loading...

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Conjugation notes

Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Secondary antibodies >> anti-Mouse >> IgM >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgM

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC/IF

     ICC/IF: 1/500 - 1/5000.

    ICC/IF: 1/500 - 1/5000.

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

  • Dot Blot

     Dot: 1/5000 - 1/100000.

    Dot: 1/5000 - 1/100000.

  • WB

     WB: 1/2000 - 1/10000.

    WB: 1/2000 - 1/10000.

  • ELISA

     ELISA: 1/90000

    ELISA: 1/90000

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    Electron Microscopy

     EM: Use at an assay depe...Read more →

    EM: Use at an assay dependent dilution.

  • IHC

     IHC: 1/500 - 1/5000.

    IHC: 1/500 - 1/5000.

References for Goat anti-Mouse IgM mu chain (HRP) secondary antibody (ab5930)

This product has been referenced in:

  • Orihuela CJet al. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. J Clin Invest : (2009). IHC-Fr; Mouse.Read more (PubMed: 19436113) »
  • Shanley DK & Sullivan AM Expression of the cell surface markers mAb 2F7 and PSA-NCAM in the embryonic rat brain. Neurosci Lett 424:165-9 (2007). WB; Mouse.Read more (PubMed: 17723277) »

See all 2 publications for this product

Publishing research using ab5930? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"