Mouse LBP ELISA Kit (ab213876)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse LBP ELISA Kit
See all LBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 3.313ng/ml 0.237 7.1% 2 16 12.997ng/ml 0.832 6.4% 3 16 39.089ng/ml 2.111 5.4% Inter-assay Sample n Mean SD CV% 1 24 3.385ng/ml 0.244 7.2% 2 24 14.212ng/ml 1.151 8.1% 3 24 39.495ng/ml 2.804 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
1.56 ng/ml - 100 ng/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213876) is designed for the quantitative detection of Mouse LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for LBP has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse LBP amount of sample captured in plate.
-
Notes
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS dependent monocyte responses.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Mouse LBP coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Mouse LBP antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse LBP standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
-
Function
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- BPI fold containing family D, member 2
- Bpifd2
- LBP
see all -
Database links
- Entrez Gene: 16803 Mouse
- SwissProt: Q61805 Mouse
- Unigene: 218846 Mouse
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab213876 has been referenced in 5 publications.
- Pellegrini C et al. Enteric a-synuclein impairs intestinal epithelial barrier through caspase-1-inflammasome signaling in Parkinson's disease before brain pathology. NPJ Parkinsons Dis 8:9 (2022). PubMed: 35022395
- Garcia-Irigoyen O et al. Enterocyte superoxide dismutase 2 deletion drives obesity. iScience 25:103707 (2022). PubMed: 35036884
- Ganguly S et al. Nonalcoholic Steatohepatitis and HCC in a Hyperphagic Mouse Accelerated by Western Diet. Cell Mol Gastroenterol Hepatol 12:891-920 (2021). PubMed: 34062281
- Yu M et al. Anti-Hyperlipidemia and Gut Microbiota Community Regulation Effects of Selenium-Rich Cordyceps militaris Polysaccharides on the High-Fat Diet-Fed Mice Model. Foods 10:N/A (2021). PubMed: 34681302
- Chivero ET et al. Cocaine Induces Inflammatory Gut Milieu by Compromising the Mucosal Barrier Integrity and Altering the Gut Microbiota Colonization. Sci Rep 9:12187 (2019). PubMed: 31434922