Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

Overview

  • Product nameMouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29)
  • Description
    Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29)
  • Product overviewab93759 is a Mouse Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 rat monoclonal, 50µg of CD90 rat monoclonal, 50µg Sca-1 rat monoclonal, 50µg of CD45 rat monoclonal and 50µl of CD29 rabbit monoclonal antibodies.
    The Mouse Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated mouse multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD44, CD29, Sca-1, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.
  • Components

      Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) images

      • IHC image of ab25340 staining CD44 on Mouse Tissue sections (T-cell lymphoma). The cells were 4% PFA fixed (10 min) and then incubated in Perkin-Elmer TNB Blocking Reagent at 0.5% as blocking agent for 30 mins at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25340, 1/250 dilution) overnight at +4°C. The secondary antibody was ab7097.
      • ICC/IF image of ab52971 staining CD29 in Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody can be used as a marker of CD29 positive human or mouse Mesenchymal Stromal Cells.
      • ICC/IF image of ab51317 staining Sca-1 in mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

      References for Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

      This product has been referenced in:
      • Chen TT  et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). Read more (PubMed: 20176926) »
      • Berggreen E  et al. Characterization of the dental lymphatic system and identification of cells immunopositive to specific lymphatic markers. Eur J Oral Sci 117:34-42 (2009). Read more (PubMed: 19196316) »

      See all 5 Publications for this product

      Product Wall

      Thank you very much for your interest in Abcam.

      Most of the antibodies in this panel has been tested for IHC-P, but not ab3105 against CD90/Thy1. If you wish to try this one for IHC-P you can use the dilution for IHC-Fr, 1-10 ug/ml. You can li...

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      Thank you for your reply.

      I would be happy to issue you a discount code for ab51317 to test in flow cytometry, the discount code is below.

      As for shipping, we ship all of our products on an ice pack and try to keep them as cold as pos...

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      Thank you for your reply.

      To clarify, will you be placing the order for this product? I ask, as we have to issue the discount code to the person placing the actual order. So if your collaborator is placing the order, then we will need to issue...

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      Thank you for your reply.

      3 of the 4 antibodies in the kit have are guaranteed to work in flow cytometry on mouse tissue. However, the component antibody ab51317 (anti-Sca1) is untested in this application and so we cannot guarantee that it wi...

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      Thank you for contacting Abcam.

      ab93759 is a Mouse Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 rat monoclonal, 50µg of CD90 rat monoclonal, 50µg Sca-1 rat monoclonal, 50µg of CD45 rat monoclonal and 50...

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      Thank you fopr your inquiry.

      This panel was compiled because all antibodies can be used in IHC.

      I agree that all antibodies apart from ab51317 (Anti-Sca1 / Ly6A/E antibody [E13 161-7])are tested and guaranteed for flow cytometry and...

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      Thank you for contacting us.


      I have checked the antibodies about suitability with flow, and except the ab51317 all are tested for this application.

      As always, if ab51317 should have been unsuitable, we would have mentioned th...

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      Thank you for your inquiry.

      Our Mesenchymal Stromal Cell Marker Panels (human: ab93758, mouse: ab93759) consist of five un-conjugated primary antibodies that can be used to characterise mesenchymal stromal cells.

      The antibodies are ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"