• Product name
    Mouse PP2A-alpha Matched Antibody Pair Kit
    See all PP2A-alpha kits
  • Detection method
  • Assay type
    ELISA set
  • Range
    125 pg/ml - 8000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PP2A-alpha.

    Both capture and detector antibodies are rabbit monoclonal antibodies.

    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.

    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.

    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab209878) which uses the same antibody pair.

    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse PP2A-alpha Capture Antibody 1 x 100µg 1 x 20µg
    Mouse PP2A-alpha Detector Antibody 1 x 25µg 1 x 5µg
    Mouse PP2A-alpha Lyophilized Protein 1 vial 1 vial
  • Research areas
  • Function
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt
  • Alternative names
    • PP2A alpha
    • PP2A-alpha
    • PP2Aalpha
    • PP2Ac
    • PP2CA
    • PP2Calpha
    • PPP2CA
    • Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform
    • Protein phosphatase 2 catalytic subunit alpha isoform
    • Protein phosphatase 2, catalytic subunit, alpha isozyme
    • Protein phosphatase 2A catalytic subunit, alpha isoform
    • Replication protein C
    • RP C
    • RP-C
    • RPC
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab218174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.


  • Standard calibration curve. Background subtracted values are graphed.



ab218174 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218174.
Please use the links above to contact us or submit feedback about this product.


Sign up