Mouse SDF1 ELISA Kit (ab100741)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 9.38 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SDF1 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 3 pg/ml -
Range
9.38 pg/ml - 600 pg/ml -
Recovery
99 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 99.34 89% - 107% Serum 99 91% - 107% Plasma 100.36 90% - 107% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SDF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100741) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum, plasma and cell culture supernatants. (Mouse SDF1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse SDF1 2 vials Recombinant Mouse SDF1 Standard (lyophilized) 2 vials SDF1 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry. -
Alternative names
- C-X-C motif chemokine 12
- Chemokine (C X C motif) ligand 12
- CXCL12
see all
Images
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SDF1 measured in culture supernatants from control or activated cells (P/L), stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours, and in biological fluids (duplicates +/- SD). All samples tested undiluted.
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Standard curve with background signal subtracted (duplicates; +/- SD).
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Representative Standard Curve using ab100741
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Representative Standard Curve using ab100741
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab100741 has been referenced in 11 publications.
- Schloss MJ et al. B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis. Nat Immunol 23:605-618 (2022). PubMed: 35352063
- Mai CL et al. CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain. Theranostics 11:1059-1078 (2021). PubMed: 33391521
- Reguilón MD et al. Voluntary wheel running protects against the increase in ethanol consumption induced by social stress in mice. Drug Alcohol Depend 212:108004 (2020). PubMed: 32408137
- Xu S et al. CXCR7 promotes melanoma tumorigenesis via Src kinase signaling. Cell Death Dis 10:191 (2019). PubMed: 30804329
- Khezri K et al. Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers. Artif Cells Nanomed Biotechnol 47:980-988 (2019). PubMed: 30857435