Overview

  • Product name
    Mouse TIMP2 ELISA Kit
    See all TIMP2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    21 pg/ml
  • Range
    47 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99 92% - 103%
    Cell culture media 118 116% - 119%
    Heparin Plasma 103 102% - 105%
    Citrate Plasma 94 86% - 100%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: RatDoes not react with: Cow
  • Product overview

    TIMP2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TIMP2 protein in mouse serum, plasma, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    TIMP2 (Tissue inhibitor of metalloproteinases 2, Metalloproteinase inhibitor 2) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding tightly to MMPs (in a 1:1 stoichiometry) and inactivating them by binding to their catalytic zinc cofactor. It is a soluble factor found circulating in serum and plasma. TIMP2 appears also have functions that are independent of MMP inhibition, including involvement in EGF signaling pathways.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab227893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (heparin) 1:200, and plasma (citrate) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 519 ng/mL in serum, 548 ng/mL in plasma (heparin) and 448 ng/mL in plasma (citrate).

  • The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleenocyte cell culture supernatant 25% and mouse lung cell culture supernatant 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 9.8 ng/mL in spleenocyte cell culture supernatant and 78.9 ng/mL in lung supernatant.

Protocols

References

ab227893 has not yet been referenced specifically in any publications.

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