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Mouse and Rabbit Specific AP (ABC) Detection IHC Kit (ab93695)

Overview

  • Product nameMouse and Rabbit Specific AP (ABC) Detection IHC Kit
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

     

    Reacts with Mouse & Rabbit IgG (H+L).

  • Tested applicationsIHC-Pmore details

Properties

  • RelevancePolyvalent / Alkaline Phosphatase is a labeled streptavidin-biotin immunoenzymatic antigen detection system.

Applications

Our Abpromise guarantee covers the use of ab93695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Mouse and Rabbit Specific AP (ABC) Detection IHC Kit images

  • Immunohistochemical staining of human esophageal carcinoma with ab40084 at a dilution of 1/200 and detected using ab93695.

  • Immunohistochemical staining of human tonsil with ab16667 at a dilution of 1/200 and detected using ab93695.

Protocols

References for Mouse and Rabbit Specific AP (ABC) Detection IHC Kit (ab93695)

ab93695 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Bone tissue samples from 9 months old female Wistar rats were collected and fixed in 4% paraformaldehyde for 72h and further decalcified in 10% EDTA in PBS (w/v) for 3 weeks at 4ºC. After mounted on paraffin blocks, 6um thick sections were cut and mounted on superfrost slides and allowed to dry at 40ºC in an oven for 1 week. Sections were then deparaffinized, rehydrated and incubated for 45 min at 70ºC in Tris-EDTA 0.05% Tween 20 pH 9.0 for antigen retrieval. After washing 3x in buffer, sections were blocked with Protein Block (Ab93695) for 20 min at RT and then incubated with Anti-4 Hydroxynonenal antibody (ab46545) for 13h at 4ºC diluted 1:200 in 1% TBS-T. Sections were further washed in TBS-T and incubated with Biotinylated Goat Anti-Polyvalent antibody (Ab93695) for 20 min at RT, further washed and incubated with Streptavidin Alkaline Phosphatase (Ab93695) for another 20 min at RT. Sigma Fast (Sigma, F4523) was used as substrate for the detection of alkaline phosphatase activity. Sections were counterstained with Mayers Hematoxilin for 1 min and mounted with aqueous mounting medium (CC/Mount, Sigma).
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Submitted Sep 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"