Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)

Overview

  • Product nameMouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)See all Polyvalent HRP kits ...
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

     

    Reacts with Mouse and Rabbit IgG (H+L)

  • Tested applicationsIHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 60 ml 125 ml
    Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml
    Protein block 1 x 60ml 1 x 125ml
    Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml
  • Research Areas
  • RelevancePolyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates.
  • Alternative names
    • Polyvalent/HRP

Applications

Our Abpromise guarantee covers the use of ab93697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) images

  • Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.

Protocols

References for Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)

This product has been referenced in:
  • McGee-Lawrence ME  et al. Sclerostin deficient mice rapidly heal bone defects by activating ß-catenin and increasing intramembranous ossification. Biochem Biophys Res Commun 441:886-90 (2013). IHC-P . Read more (PubMed: 24211207) »

See 1 Publication for this product

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Thank you for contacting us. The kit contains Protein Block (60ml), Anti-Polyvalent Plus (60ml) and Streptavidin Peroxidase Plus (60ml). Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"