For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
|Components||60 ml||125 ml|
|Biotinylated Goat Anti-Polyvalent Plus||1 x 60ml||1 x 125ml|
|Protein block||1 x 60ml||1 x 125ml|
|Streptavidin Peroxidase Plus||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab93697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
Publishing research using ab93697 ? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"