Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)

Overview

  • Product nameMouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
    See all HRP/AEC Detection Kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

     

    Reacts with Mouse and Rabbit IgG (H+L)

  • Tested applicationsIHC-Pmore details

Properties

  • RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names
    • carbazole
    • Horseradish Peroxidase

Applications

Our Abpromise guarantee covers the use of ab93705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit images

  • Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).

Protocols

References for Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)

ab93705 has not yet been referenced specifically in any publications.

Product Wall

CD34 detection using HRP/AEC Detection Kit (ab93705).

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human cornea stained with anti-CD34 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Fig.: Immunohistochemistry (Cryosections fixed in cold acetone) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705).

Stanislava Reinstein Merjava
Laboratory of the Biology and Pathology of the Eye
Institute of Inherited Metabolic Disorders
Charles University, First Faculty of Medicine and General University Hospital
Username

Stanislava Reinstein Merjava

Verified customer

Submitted Oct 06 2015



Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"