Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696)

Overview

• Product nameMouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP)See all IgG Light chain secondary antibodies ...
• Target speciesRabbit
• Specificityab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD).
• Tested applicationsELISA, WB more details
• Immunogen

  Rabbit Light Chain

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 50% Glycerol, 50mM Tris, 1mM Magnesium chloride, pH 8
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberSB62a
• IsotypeIgG2b
• Light chain typelambda
• Research Areas
  • Secondary antibodies
  • anti-Rabbit
  • IgG Light chain
  • Enzyme
  • Alkaline Phosphatase

Applications

Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) images

• 

  Western blot - Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696)

  Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains

  
  

  Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)

  Lane 1: 10 µg rabbit monoclonal was loaded
  Lane 2: 5 µg rabbit monoclonal was loaded
  Lane 3: 2 µg rabbit monoclonal was loaded
  

  Right blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000

  Lane 1: 1 µg rabbit monoclonal was loaded
  Lane 2: 0.5 µg rabbit monoclonal was loaded
  Lane 3: 0.25 µg rabbit monoclonal was loaded

• 

  Western blot - Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696)
  All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
  
  Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
  Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
  Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.

  Predicted band size : 42 kDa

  Observed band size : 42 kDa
  
  Exposure time : 12 minutes