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Mouse on Mouse Polymer IHC Kit (ab127055)

Q&A (15)

Overview

  • Product nameMouse on Mouse Polymer IHC Kit
  • Sample type
    Tissue
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab127055 is a robust ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background. The antibody-antigen interaction is visualized by adding a HRP conjugated secondary antibody followed by a chromogen (e.g. DAB (ab94665)) that yields a permanent brown precipitate. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods and improves signal-to-noise ratios.

  • Notes

    Additional materials required:

    • Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
    • Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab94677)
    • Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab94680)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab94681)
    • DAB substrate (ab94665)
  • Tested applicationsIHC-P more details

Properties

  • Components 15 ml 60 ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml
    Mouse on Mouse HRP Polymer 1 x 15ml 1 x 60ml
    Rodent Block 1 x 15ml 1 x 60ml
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab127055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P

Mouse on Mouse Polymer IHC Kit images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Mouse MRP2 antibody staining MRP2 in mouse liver with the use of the ab127055.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Mouse Pan CK (Clone AE1/AE3) antibody staining Pan CK in mouse colon with the use of ab127055.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse on Mouse Polymer IHC Kit (ab127055)
    Mouse Pax-5 antibody staining Pax-5 in mouse spleen with the use of the ab127055.

Protocols

References for Mouse on Mouse Polymer IHC Kit (ab127055)

ab127055 has not yet been referenced specifically in any publications.

Publishing research using ab127055 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab127055

Displaying 1 - 10 of 15 results for Q&A

10
Votes: Highest First

Dear technical team,

Our customer would like to purchase several reagents for IHC setting.
He has only mouse host antibodies now but,there's a possibility to use rabbit host antibody additionally, I recommended ab80436.
But I'm confus...

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Thank you for your enquiry and your interest in our products.

I understand from your previous e-mail that your customer will perform IHC-P with samples from either rat or rabbit.

I can confirm that ab80436 EXPOSE Mouse and Rabbit Speci...

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Customer kindly contacted us regarding ab7032.

Thank you for contacting us.

As requested, I have attached the datasheet for ab7032 as well as our Mouse on Mouse staining protocol. Abcam also carries a Mouse on Mouse staining kit which contains all of the reagents need to perform mouse on ...

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Thank you for your reply.

Is this mean the secondary antibody in the kit reacts with mouse IgM antibodies also? Generally, the IHC kits include anti-IgG secondary antibodies. I would like to be sure exactly; without misunderstanding.

Hav...

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Thank you for contacting us.

This kit will work with mouse IgM antiobodies as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclon...

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I would like to be sure that Mouse on Mouse Polymer IHC Kit (ab127055) is suitable for the fallowing antibodies (IHC-P on mouse tissue)

TFF 2 antibody / Spasmolytic Polypeptide [GE16C] (ab49536) 500 µg, Mouse monoclonal IgM

Anti-Tr...

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Thank you for contacting us.

ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human samples ...

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Customer kindly contacted us to find an anti-IL10 product to use in IHC-P/F in mouse. This product cannot react to human IL-10.

Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around aa1...

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Guten Tag,
wir haben vor einiger Zeit bei Ihnen den ab2378 AK bestellt und ihn, wie damals im Datasheet stand, bei 4 Grad gelagert. Nun ist der Antikörper nicht mehr funktionsfähig, wie ein Test zeigte. In aktuellen Datasheets steht, dass ma...

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Vielen Dank für Ihre Anfrage.

Gerne senden wir Ihnen einen kostenlosen Ersatz. Bitte teilen Sie mir doch mit, wann (ungefähr) Sie den Antikörper gekauft haben, damit ich Ihre Bestellung heraus suchen kann.

Wenn Sie eine &q...

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mouse liver, IHC-P, high background in endothelial cells. Paraffin embedded sections, negative control also showing staining, secondary antibody is fine. H202 block 15 min, protein block 5 min

Thank you for contacting us.

You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative cont...

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The customer below would like to know if Abcam has an antibody to stain proteins or antigens of MC-38 cells. This is a type of murine colon carcinoma.

I couldn’t find anything on our website or on biocompare. I’m wondering if this pro...

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Thank you for contacting Abcam.

I have searched our catalogue, but cannot find any markers specific to MC-38 cells. Do you of any of any specific proteins that are only expressed in these types of cells? As knowing this, would help us find a suit...

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I am writing mail to confirm OX2R antibody is identical to CD200R antibody.
I bought CD200 antibody (Ab93315). It works very well in frozen
section of mouse.
Furthermore, I am going to want to stain CD200 with CD200R doubly.
However I ...

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Thank you for contacting us.

I have checked the SwissProt page for the OX-2 membrane glycoprotein, and it looks it is a ligand for CD200R1.Therefore, ab104701 is not the antibody I would like to recommend for your experiments.

However...

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I refer to your Anti-Sarcomeric Alpha Actinin antibody [EA-53] antibody.

Currently I am using paraffin mouse muscle sections and would like to label Z
bands
together with Myosin (MF20 a general muscle marker monoclonal mouse).
Howev...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"