Overview

  • Product nameMouse on Mouse Polymer IHC Kit
  • Sample type
    Tissue
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab127055 is a robust ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background. The antibody-antigen interaction is visualized by adding a HRP conjugated secondary antibody followed by a chromogen (e.g. DAB (ab94665)) that yields a permanent brown precipitate. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods and improves signal-to-noise ratios.

  • Notes

    Additional materials required:

    • Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674)
    • Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab94677)
    • Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) (ab94680)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab94681)
    • DAB substrate (ab94665)
  • Tested applicationsSuitable for: IHC-Pmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab127055 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent concentration.

    Mouse on Mouse Polymer IHC Kit images

    • Mouse Pan CK (Clone AE1/AE3) antibody staining Pan CK in mouse colon with the use of ab127055.
    • Mouse Pax-5 antibody staining Pax-5 in mouse spleen with the use of the ab127055.

    Protocols

    References for Mouse on Mouse Polymer IHC Kit (ab127055)

    This product has been referenced in:
    • Song D  et al. Retinal Pre-Conditioning by CD59a Knockout Protects against Light-Induced Photoreceptor Degeneration. PLoS One 11:e0166348 (2016). IHC-P ; Mouse . Read more (PubMed: 27893831) »
    • He J  et al. A Developmental Stage-Specific Switch from DAZL to BOLL Occurs during Fetal Oogenesis in Humans, but Not Mice. PLoS One 8:e73996 (2013). Read more (PubMed: 24086306) »

    See all 3 Publications for this product

    Product Wall

    Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with a 50/50 mixture of Rodent Block and 3% BSA in 0.1% Triton X-100 + PBS. Sections were then incubated for 1h at RT with either (A) no primary antibody or (B) anti-DDX4 (Abcam ab27591) at a 1:100 dilution in a 50/50 mixture of Rodent Block and 3% BSA in 0.1% Triton X-100 + PBS. Sections were then washed 3X with 0.1% Triton X-100 in PBS. Donkey Anti-Mouse 555 (Abcam ab150110) applied at a 1:500 dilution in 3% BSA in 0.1% Triton X-100 + PBS. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Nov 21 2016

    Thank you for your enquiry and your interest in our products.

    I understand from your previous e-mail that your customer will perform IHC-P with samples from either rat or rabbit.

    I can confirm that ab80436 EXPOSE Mouse and Rabbit Sp...

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    Thank you for contacting us.

    As requested, I have attached the datasheet for ab7032 as well as our Mouse on Mouse staining protocol. Abcam also carries a Mouse on Mouse staining kit which contains all of the reagents need to perform mouse ...

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    Thank you for contacting us.

    This kit will work with mouse IgM antiobodies as well.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoc...

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    Thank you for contacting us.

    ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

    Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

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    Thank you for contacting us.

    I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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    Vielen Dank für Ihre Anfrage.

    Gerne senden wir Ihnen einen kostenlosen Ersatz. Bitte teilen Sie mir doch mit, wann (ungefähr) Sie den Antikörper gekauft haben, damit ich Ihre Bestellung heraus suchen kann.

    Wenn Sie eine...

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    Thank you for contacting us.

    You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative c...

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    Thank you for contacting Abcam.

    I have searched our catalogue, but cannot find any markers specific to MC-38 cells. Do you of any of any specific proteins that are only expressed in these types of cells? As knowing this, would help us find a s...

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    Thank you for contacting us.

    I have checked the SwissProt page for the OX-2 membrane glycoprotein, and it looks it is a ligand for CD200R1.Therefore, ab104701 is not the antibody I would like to recommend for your experiments.

    Howe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"