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ab127055 is a robust ONE-step polymer system that provides increased sensitivity, time savings and detection simplicity. ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background. The antibody-antigen interaction is visualized by adding a HRP conjugated secondary antibody followed by a chromogen (e.g. DAB (ab94665)) that yields a permanent brown precipitate. This system is also biotin-free, which eliminates background staining found with traditional biotin-based detection methods and improves signal-to-noise ratios.
Additional materials required:
|Components||15 ml||60 ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 60ml|
|Mouse on Mouse HRP Polymer||1 x 15ml||1 x 60ml|
|Rodent Block||1 x 15ml||1 x 60ml|
Our Abpromise guarantee covers the use of ab127055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemical analysis with ab127055 showing Dazl and Boll protein expression during mouse fetal ovary development.
High expression of Dazl (top panel) is seen in germ cells through all stages of development. Boll (bottom panel) is only expressed in germ cells during e15.5.
Immunohistochemical analysis of mouse eye using ab127055 to optimize signal to background staining ratio.