Mouse specific HRP/DAB detection IHC kit (ab64259)

Would like to test antibody in IHC-P.

ANSWER:

DISCOUNT CODE: XXXXXXXXXXX
Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab89566 in [IHC-P]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [IHC-P] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Bonjour,

Tout d'abord merci pour votre aide.

J'ai effectué les tests avec votre kit de révélation pour la NGF et la TRkA avec les paramètres suivant :

- 3*5min a 600w dans un tampon à pH9

- 30 min de peroxydase

- 1h de serum block

- 30 min anticorps primaire

- 15 min biotine

- 15 min streptadivine

- DAB, Hematoxyline etc.

Nous avons tester sur le cerveau, sur la peau et sur de la muqueuse gingival. Nous avons un marquage mais nous ne sommes pas sur du résultat car dans la muqueuse l'épithélium est intégralement marqué. Est ce normal ?
De plus notre contrôle négatif, qui vient de chez vous,(ab47472) à aussi un léger font.

Les conditions si-dessus sont celle qui on donné le meilleur résultat. Pouvons nous vous envoyer les coupe afin que vous nous donniez votre avis ?

Merci d'avance pour votre aide.

ANSWER:

Merci pour votre émail.

Je suis désolée que les résultats ne sont pas claires.

Je vais volontiers investiguer ces résultats et essayer de donner des conseils pour améliorer les images. Si un de nos produits serait défectueux, nous le remplacerons en accord avec notre garantie.

Pour mieux comprendre la problème, est-ce que vous pouvez s'il vous plaît m'envoyer les images annotées de ces résultats? C'est plus pratique si vous pouvez nous envoyer les images, et non les slides.

Aussi, pourriez vous s'il vous plait dire quels anticorps exactement vous avez utilisés pour les marquages avec quels kits? C'était le ab6199 ou le ab3125?

Est-ce que vous avez utilisé des coupes paraffine de souris? Quel sérum est-ce que vous avez utilisé pour le blocage? Est-ce que vous avez aussi utilisé le protéine block du kit?

Je me réjouis de votre réponse.

Thank you.

I will follow your links.

I asked about prices because I might have the order via relatives in Canada or China.

Many thanks.

ANSWER:

Thank you for your reply.

If you change the country at the top right hand site of the Abcam website (http://www.abcam.com), you can see the country specific prices and also (in case of China) the distributor details.

Please be aware that we might not be able to ship to residential addresses.

I wish you good luck with your research.

Dear Sir,

Good morning,

Kindly advise me about Mouse specific HRP/DAB detection IHC kit (ab64259).

I need to know how many samples can be stained using a single kit.

Kindly inform me about the overall cost including the kit price, shipping cost, additional fees. I live in Egypt.

You can simply consider shipping address to International Cairo Airport, I should receive the kit to pay taxes.

I will need one antibody or more, but I have to decide which one to choose.

Many thanks.

ANSWER:

Thank you for your inquiry.

The reagents of this kit have to be used according to the size of the samples.

If you are using 100ul of the DAB mixed with DAB substrate per slide(this depends on the size of the sample on the slide, it needs to be covered all the way) then one kit of ab64259 will be enough for250 slides.

Our datasheet gives all the information about the amount of the components of the kit to calculate this according to your experimental needs. Please follow this link to review the datasheet:

http://www.abcam.com/index.html?datasheet=64259 (or use the following: http://www.abcam.com/index.html?datasheet=64259).

We also do not ship directly to Egypt. Please contact our trusted distributor KEMET Medical for information on pricing and shipping.

KEMET Medical
14a El Emam Aly St
Off Orouba St - Ismailia Square
Heliopolis 11341, Cairo

Egypt
Tel 26903127, 22903301 Website http://www.kemet.com.eg/docs/medical.aspx Fax 26903126 Email mailto:mrashidy@kemet.com.eg

I hope this information was helpful and wish you good luck with their research.

Will this secondary react with rat IgG1?

ANSWER:

Thank you for contacting us.

Cross-reactivity of anti-mouse IgG ab64259 with rat IgG has not been tested, but at least it has not been absorbed to remove reactivity with rat. So, the short answer is that we do not know if the secondary will be reactive with rat. The long answer is that we assume it will react with rat IgG, but we recommend a test before committing to a large run of samples.

Please do not hesitate to contact us if you need any more advice or information.