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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Mov10 antibody
See all Mov10 products (3) ...
Description
Rabbit polyclonal to Mov10
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Cow, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human Mov10.
(Peptide available as ab28937.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Immunocytochemistry/ Immunofluorescence - Mov10 antibody (ab28656)
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Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab28937).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
Cellular localization
Cytoplasmic
Alternative names
- DKFZp667O1423 antibody
- FLJ32791 antibody
- fSAP113 antibody
see all
Database links
- Entrez Gene: 4343 Human
- Entrez Gene: 17454 Mouse
- Omim: 610742 Human
- SwissProt: Q9HCE1 Human
- SwissProt: P23249 Mouse
Anti-Mov10 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - Mov10 antibody (ab28656)
ICC/IF image of ab28656 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28656, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-Mov10 antibody (ab28656)
ab28656 has not yet been referenced specifically in any publications.
Publishing research using ab28656? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"