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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab60132? Please let us know so that we can cite the reference in this datasheet
ab60132 has been referenced in 1 publications.
- Messaoudi-Aubert SE et al. Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a tumor suppressor. Nat Struct Mol Biol 17:862-8 (2010). WB, ICC/IF; Human. PubMed: 20543829
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Mov10 antibody (ab60132)
Anti-Mov10 antibody (ab60132) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.
Predicted band size : 114 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Mov10 antibody (ab60132)
Human liver tissue stained with ab60132 at 4-8 µg/ml. The arrows point to hepatocytes (positive labeling of Mov10).
Immunocytochemistry/ Immunofluorescence-Mov10 antibody(ab60132)
ICC/IF image of ab60132 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60132, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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