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I have used this antibody in mouse and it did not work. Can I get a refund? |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Mov10 antibody (ab60132) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.
Predicted band size : 114 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Human liver tissue stained with ab60132 at 4-8 µg/ml. The arrows point to hepatocytes (positive labeling of Mov10).
ICC/IF image of ab60132 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60132, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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