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Western blot - Mov10 antibody (ab60132)
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Immunohistochemistry (Paraffin-embedded sections) - Mov10 antibody (ab60132)
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Product Name
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Mov10 antibody
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See all Mov10 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Mov10
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Immunogen
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A region within synthetic peptide: FCKENGGYTG CPFPAKLDLQ QGQNLLQGLS KLSPSTSGPH SHDYLPQERE, corresponding to C terminal amino acids 937-986 of Human Mov10
BLAST 'FCKENGGYTG CPFPAKLDLQ QGQNLLQGLS KLSPSTSGPH SHDYLPQERE ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:312500
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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MOV10 may be an helicase with an important function in development and/or control of cell proliferation.
RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mov10 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Mov10 antibody images, references, products related to ab60132 and other tools.
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Mov10 antibody images:
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 Western blot - Mov10 antibody (ab60132)
Mov10 antibody (ab60132) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.
Predicted band size : 114 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - Mov10 antibody (ab60132)
This image shows human liver tissue stained with ab60132 at 4-8 µg/ml. The arrows point to hepatocytes (positive label).
Human liver tissue stained with ab60132 at 4-8 µg/ml. The arrows point to hepatocytes (positive labeling of Mov10).
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Search PubMed (MEDLINE) for references to Mov10
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Mov10 antibody - more information
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Customer reviews (feedback) regarding Mov10 antibody |
Customer FAQs regarding Mov10 antibody |
Protocols for Mov10 antibody |
Price and availability of products related to Mov10 antibody |
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Mov10 antibody - related products:
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Mov10 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Mov10 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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