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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Mov10 antibody
See all Mov10 products (3) ...
Description
Rabbit polyclonal to Mov10
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
A region within synthetic peptide: FCKENGGYTG CPFPAKLDLQ QGQNLLQGLS KLSPSTSGPH SHDYLPQERE, corresponding to C terminal amino acids 937-986 of Human Mov10
FCKENGGYTG CPFPAKLDLQ QGQNLLQGLS KLSPSTSGPH SHDYLPQERE
Positive control
Human liver.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 114 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
ICC/IF: Use at an assay dependent dilution. (PubMed: 20543829)
Target
Relevance
MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
Cellular localization
Cytoplasmic
Alternative names
- DKFZp667O1423 antibody
- FLJ32791 antibody
- fSAP113 antibody
see all
Database links
- Entrez Gene: 4343 Human
- Entrez Gene: 17454 Mouse
- Entrez Gene: 310756 Rat
- Omim: 610742 Human
- SwissProt: Q9HCE1 Human
- SwissProt: P23249 Mouse
Anti-Mov10 antibody images:
Western blot - Mov10 antibody (ab60132)
Anti-Mov10 antibody (ab60132) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.
Predicted band size : 114 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Mov10 antibody (ab60132)
Human liver tissue stained with ab60132 at 4-8 µg/ml. The arrows point to hepatocytes (positive labeling of Mov10).
Immunocytochemistry/ Immunofluorescence-Mov10 antibody(ab60132)
ICC/IF image of ab60132 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60132, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Mov10 antibody (ab60132)
This product has been referenced in:
- Messaoudi-Aubert SEet al. Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a tumor suppressor. Nat Struct Mol Biol 17:862-8 (2010). WB, ICC/IF; Human.Read more (PubMed: 20543829) »
See 1 publication for this product
Publishing research using ab60132? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"