Anti-Mov10 antibody (ab80613)

We have ordered a couple of weeks ago 3 Abs for matrin3, TRBP2 and Mov10 proteins. We used those Abs for WB and none of them worked. TRBP2 (AB72110) it doesnt give any band after o/n or 2-3h RT even in 1/500 dilution and matrin3 (ab51081) Mov10 (ab80613) they really give many many bands but not the right ones.

These Abs came together, is it possible that sth happen during transfer? Would you please help us with make them work? Alternatively, could you please consider change them with another lot that you know is working or another Ab for these mouse proteins?

We are looking forward for your reply in your earliest convenience!! Thank you in advance!!

ANSWER:

Thank you for contacting us.

I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and any troubleshooting steps which you have performed? After review of these details I may be able to offer recommendations which would improve the results which you have been experiencing. Should we not be able to improve these results, these products are covered by our Abpromise guarantee. We provide replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

http://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I was wondering if I could get more information about this antibody (Mov10 Antibody ab80613).  Has anyone used it successfully for a co-immunopreceipitation experiment?   Thanks,    

ANSWER:

Thank you for your inquiry.

Unfortunately, we do not know of a publication where this antibody was used in a co-IP experiment.

I would like to reassure you that this antibody is tested and guaranteed for IP by our Abpromise.

To co-IP a protein the protocol might have to be adjusted from normal IP.

I can recommend to review our Protocol section and the recent webinar about IP from one of our IP experts. Please follow these links:

http://www.abcam.com/index.html?pageconfig=resource&rid=11385

http://dev-pws1.abcam.com/blog/index.cfm/2011/11/17/Immunoprecipitation-Procedurespitfalls-and-protocol-tips

I hope you will find this information helpful. Please do not hesitate to contact us with any further questions.