Anti-Mov10 antibody (ab80613)

  Western blot - Mov10 antibody (ab80613)

All lanes : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg

developed using the ECL technique

Predicted band size : 114 kDa
Observed band size : 114 kDa


Exposure time : 3 minutes

  Immunoprecipitation - Mov10 antibody (ab80613)

Immunoprecipitation of HeLa whole cell lysate using ab80613 at 3µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.

  Immunocytochemistry/ Immunofluorescence-Mov10 antibody(ab80613)

ICC/IF image of ab80613 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80613, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.