Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
Key features and details
- Mouse monoclonal [N1] to TTK/Mps1 - BSA free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TTK/Mps1 antibody [N1] - BSA free
See all TTK/Mps1 primary antibodies -
Description
Mouse monoclonal [N1] to TTK/Mps1 - BSA free -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TTK/Mps1 aa 1-900. The exact sequence is proprietary. (catalytically inactive - D663A)
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Epitope
This antibody recognizes an epitope in the N-terminal region of Mps1. -
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N1 -
Myeloma
PAIB3Ag81 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour. |
Target
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Function
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint. -
Tissue specificity
Present in rapidly proliferating cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 7272 Human
- Omim: 604092 Human
- SwissProt: P33981 Human
- Unigene: 169840 Human
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Alternative names
- cancer/testis antigen 96 antibody
- CT96 antibody
- Dual specificity protein kinase TTK antibody
see all
Images
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All lanes : Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
NOTE: MILK was used to block non-specific binding, rather than BSA. -
Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +
Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Exposure time: 8 minutesab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab11108 has been referenced in 25 publications.
- Sarwar S et al. Inhibition of Mps1 kinase enhances taxanes efficacy in castration resistant prostate cancer. Cell Death Dis 13:868 (2022). PubMed: 36229449
- Fang Q et al. The essential roles of Mps1 in spermatogenesis and fertility in mice. Cell Death Dis 12:531 (2021). PubMed: 34031364
- Xia P et al. Reversine inhibits proliferation, invasion and migration and induces cell apoptosis in gastric cancer cells by downregulating TTK. Exp Ther Med 22:929 (2021). PubMed: 34306198
- Lu N & Ren L TTK (threonine tyrosine kinase) regulates the malignant behaviors of cancer cells and is regulated by microRNA-582-5p in ovarian cancer. Bioengineered 12:5759-5768 (2021). PubMed: 34516342
- Lu L et al. Targeting Mps1 in combination with paclitaxel inhibits osteosarcoma progression by modulating spindle assembly checkpoint and Akt/mTOR signaling. Oncol Lett 22:797 (2021). PubMed: 34584572