Anti-Mre11 antibody [44B12] (ab119704)

Overview

• Product nameAnti-Mre11 antibody [44B12]See all Mre11 primary antibodies ...
• Description
  Mouse monoclonal [44B12] to Mre11
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant Human Mre11 with His tag, purified from E.coli.

• Positive controlHeLa, SK-N-MC, K562, A431, HepG2, SK-BR3 and Ramos cell lysates.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.03% Sodium azide
  Constituents: 49% HEPES, 50% Glycerol, 0.88% Sodium chloride, 0.01% BSA
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Clonality Monoclonal
• Clone number44B12
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Telomeres

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Non Homol. End Joining

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

Applications

Target

• FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
• Involvement in diseaseDefects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
• Sequence similaritiesBelongs to the MRE11/RAD32 family.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.

Target information above from: UniProt accession P49959 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4361 Human
  • Entrez Gene: 17535 Mouse
  • Entrez Gene: 64046 Rat
  • Omim: 600814 Human
  • SwissProt: P49959 Human
  • SwissProt: Q61216 Mouse
  • SwissProt: Q9JIM0 Rat
  • Unigene: 192649 Human

  • Unigene: 149071 Mouse
  • Unigene: 209040 Rat

  see all

• Alternative names
  AT like disease antibodyAtaxia telangiectasia disorder like antibodyATLD antibody

  DNA recombination and repair protein antibodyDouble strand break repair protein MRE11A antibodyDouble-strand break repair protein MRE11A antibodyendo/exonuclease Mre11 antibodyHNGS1 antibodymeiotic recombination (S. cerevisiae) 11 homolog A antibodyMeiotic recombination 11 homolog 1 antibodymeiotic recombination 11 homolog A (S. cerevisiae) antibodyMeiotic recombination 11 homolog A antibodyMmMRE11A antibodyMre 11 antibodyMRE 11a antibodyMRE 11b antibodyMRE11 homolog 1 antibodyMRE11 homolog A antibodyMRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibodyMRE11 meiotic recombination 11 homolog A antibodyMRE11_HUMAN antibodyMRE11A antibodyMRE11b antibodyOTTHUMP00000236830 antibodyOTTHUMP00000236831 antibodyOTTHUMP00000236832 antibodyOTTHUMP00000236833 antibody

  see all

Anti-Mre11 antibody [44B12] images

• 

  Western blot - Anti-Mre11 antibody [44B12] (ab119704)
  All lanes : Anti-Mre11 antibody [44B12] (ab119704) at 1/2000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : SK-N-MC cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : A431 cell lysate
  Lane 5 : HepG2 cell lysate
  Lane 6 : SK-BR3 cell lysate
  Lane 7 : Ramos cell lysate
  
  
  Predicted band size : 81 kDa