Anti-Mucin 5AC antibody [CLH2] (ab77576)
Key features and details
- Mouse monoclonal [CLH2] to Mucin 5AC
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mucin 5AC antibody [CLH2]
See all Mucin 5AC primary antibodies -
Description
Mouse monoclonal [CLH2] to Mucin 5AC -
Host species
Mouse -
Specificity
ab77576 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6 or 7 The antibody recognises glycosylated as well as unglycosylated MUC5AC. -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CLH2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
Tissue specificity
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
Post-translational
modificationsC-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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Alternative names
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
Images
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ab77576 staining of foveolar epithelium of human gastric mucosa using IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex. Colour Development by DAB. Hematoxylin counterstained.
This image was generated using the ascites version of the product.
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ab77576 staining of foveolar epithelium of human gastric mucosa by IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex.
This image was generated using the ascites version of the product.
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Overlay histogram showing A549 cells stained with ab77576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab77576 has been referenced in 6 publications.
- Uehara K et al. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports 17:820-834 (2022). PubMed: 35245440
- Beisl J et al. Assessing Mixture Effects of Cereulide and Deoxynivalenol on Intestinal Barrier Integrity and Uptake in Differentiated Human Caco-2 Cells. Toxins (Basel) 13:N/A (2021). PubMed: 33806705
- Zhang J et al. Evaluation of serum MUC5AC in combination with CA19-9 for the diagnosis of pancreatic cancer. World J Surg Oncol 18:31 (2020). PubMed: 32028958
- Lee HY et al. Platycodi Radix and its active compounds ameliorate against house dust mite-induced allergic airway inflammation and ER stress and ROS by enhancing anti-oxidation. Food Chem Toxicol 123:412-423 (2019). PubMed: 30399386
- Sim HJ et al. Xenopus: An alternative model system for identifying muco-active agents. PLoS One 13:e0193310 (2018). PubMed: 29470529
- Kaur S et al. A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study. Am J Gastroenterol 112:172-183 (2017). PubMed: 27845339