Anti-Munc13-1 antibody - C-terminal (ab215426)
Key features and details
- Sheep polyclonal to Munc13-1 - C-terminal
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Munc13-1 antibody - C-terminal
See all Munc13-1 primary antibodies -
Description
Sheep polyclonal to Munc13-1 - C-terminal -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Munc13-1 aa 1500-1700 (C terminal). (Derived from E.coli).
Database link: Q9UPW8 -
Positive control
- Mouse embryonic tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The IgG fraction of immunized serum was purified by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 190-210 kDa (predicted molecular weight: 193 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 190-210 kDa (predicted molecular weight: 193 kDa). |
Target
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Relevance
Function: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). Also involved in secretory granule priming in insulin secretion (By similarity). Interacts with FBXO45 (via SRY domain); leading to the degradation of UNC13A by the proteasome (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Tissue specificity: Expressed in pancreatic islet cells (PubMed:12871971). Expressed in melanocytes (PubMed:23999003). Similarity: Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger. Domain: The C2 domains are not involved in calcium-dependent phospholipid binding. The C-terminal region containing both MHD domains and the third C2 domain is required for synaptic vesicle priming activity. -
Cellular localization
Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction; synapse, presynaptic cell membrane. Note: Localized to the active zone of presynaptic density. Translocated to the plasma membrane as response to phorbol ester binding -
Database links
- Entrez Gene: 23025 Human
- Entrez Gene: 22249 Mouse
- Entrez Gene: 382018 Mouse
- Entrez Gene: 64829 Rat
- Omim: 609894 Human
- SwissProt: Q9UPW8 Human
- SwissProt: Q4KUS2 Mouse
- SwissProt: Q62768 Rat
see all -
Alternative names
- KIAA1032 antibody
- Munc 13 antibody
- Munc13-1 antibody
see all
Images
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Anti-Munc13-1 antibody - C-terminal (ab215426) at 1/1000 dilution + Mouse embryonic tissue lysate
Predicted band size: 193 kDa
Observed band size: 190-210 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab215426 has not yet been referenced specifically in any publications.