Anti-Munc18c antibody (ab224625)
Key features and details
- Rabbit polyclonal to Munc18c
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Munc18c antibody
See all Munc18c primary antibodies -
Description
Rabbit polyclonal to Munc18c -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Munc18c aa 250-550.
Database link: O00186 -
Positive control
- WB: Mouse kidney tissue lysate. IHC-P: Human adrenal gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. -
Tissue specificity
Megakaryocytes and platelets. -
Sequence similarities
Belongs to the STXBP/unc-18/SEC1 family. -
Post-translational
modificationsPhosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4. -
Cellular localization
Cytoplasm > cytosol. Cell membrane. In platelets, predominantly cytosolic. Low amounts membrane-associated. - Information by UniProt
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Database links
- Entrez Gene: 6814 Human
- Entrez Gene: 20912 Mouse
- Omim: 608339 Human
- SwissProt: O00186 Human
- SwissProt: Q60770 Mouse
- Unigene: 530436 Human
- Unigene: 316894 Mouse
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Alternative names
- Munc18 3 antibody
- Munc18 c antibody
- Platelet Sec1 protein antibody
see all
Images
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Anti-Munc18c antibody (ab224625) at 1/500 dilution + Mouse kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Paraffin-embedded human adrenal gland tissue stained for Munc18c using ab224625 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224625 has not yet been referenced specifically in any publications.