Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end (ab41169)

Overview

  • Product nameAnti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal endSee all Muscarinic Acetylcholine Receptor M3 primary antibodies ...
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3 - Carboxyterminal end
  • Specificityab41169 recognises the C terminal region of Muscarinic Acetylcholine Receptor M3. We have run several ELISA based assays and found no cross reactivity between this antibody and the M1, M2, M4 and M5 receptors.
  • Tested applicationsWB, ELISA, ICC/IF, IHC-Fr more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide: FHKRVPEQAL (Rat) conjugated to KLH by a Cysteine residue linker

  • Positive control
    • PLP fixed rat brain sections, whole rat brain homogenate.

Properties

Applications

Our Abpromise guarantee covers the use of ab41169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500. Predicted molecular weight: 66 kDa.Can be blocked with Muscarinic Acetylcholine Receptor M3 peptide (ab49398). Block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. Dilute antibody in blocking buffer.With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/2500. PLP fixed.

Target

  • FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane.
  • Target information above from: UniProt accession P20309 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACM3_HUMAN antibody
    • cholinergic receptor muscarinic 3 antibody
    • CHRM 3 antibody
    • CHRM3 antibody
    • EGBRS antibody
    • HM 3 antibody
    • HM3 antibody
    • m3 muscarinic acetylcholine receptor antibody
    • M3 muscarinic receptor antibody
    • muscarinic 3 antibody
    • Muscarinic acetylcholine receptor M3 antibody
    • muscarinic cholinergic m3 receptor antibody
    • muscarinic m3 receptor antibody
    see all

Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end images

  • ICC/IF image of ab41169 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41169) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end (ab41169)

ab41169 has not yet been referenced specifically in any publications.

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Our record shows the antibody gave good signal even at 1/2500 dilution with PLP (periodate-lysine-paraformaldehyde fixative) fixed rat brain sections using ABC technique so optimization of dilution factor is highly re...

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Thank you for contacting us.

We apologize for the error; the datasheet has been updated by removing the concentration of the antibody. We recommend trying different dilutions e.g.1/100, 1/500 or 1/1000 for optimization.

I hope this in...

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I am very pleased to hear you would like to accept our offer and test ab41169 Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To re...

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Thank you very much for your interest in ab41169 Anti-Muscarinic Acetylcholine Receptor M3 antibody - Carboxyterminal end.


To our knowledge, ab41169 has not been tested in mouse.I performed a BLAST search, and itlooks like the peptide can...

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Leider haben wir keinen anti Muscarinic Acetylcholine Receptor M3 Antikörper, welcher schon auf Mausgewebe und in der Immunfluorszenz (IF) oder in Immunoh...

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I have received the following information from the laboratory which tested this antibody: " We have run several ELISA based assays and found no cross reactivity between this antibody and other non M3 receptors. Polypeptide ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"