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Synthetic peptide: FHKRVPEQAL (Rat) conjugated to KLH by a Cysteine residue linker
Our Abpromise guarantee covers the use of ab41169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 66 kDa.Can be blocked with Muscarinic Acetylcholine Receptor M3 peptide (ab49398). Block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. Dilute antibody in blocking buffer.With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||1/2500. PLP fixed.|
ICC/IF image of ab41169 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41169) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab41169 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"