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Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)

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Overview

Product name

Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2]
See all Muscarinic Acetylcholine Receptor M4 products (2) ...

Description

Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4

Specificity

ab77956 reacts with Muscarinic acetylcholine receptor M4. There is no reactivity with the other subtypes.

Tested applications

IHC-Fr, Flow Cyt, WB, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Monkey

Immunogen

Muscarinic Acetylcholine Receptor M4 i3 loop (human), fused to a tag.

Positive control

Transfected CHO cell lines

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

18C7.2

Isotype

IgG1

Research areas

Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Acetylcholine
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Muscarinic Receptors

  • Flow Cytometry-Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2](ab77956)Flow Cytometry-Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2](ab77956) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.

Cellular localization

Cell Membrane

Alternative names

  • Cholinergic receptor muscarinic 4 antibody
  • Chrm 4 antibody
  • CHRM4 antibody
  • HM 4 antibody
  • HM4 antibody
  • M4 antibody
see all

Database links

Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] images:

  Flow Cytometry-Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2](ab77956)

Flow Cytometry-Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2](ab77956)

Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)

ab77956 has not yet been referenced specifically in any publications.

Publishing research using ab77956? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"